For example, the enhanced standard may be necessary when using a 1:50,000 (25 ng/mL) to 1:100,000 (10 ng/mL) dilution of secondary antibody. SuperSignal Enhanced Molecular Weight Protein ladder is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. As originally conceived by Towbin et al., the technique enables indirect detection of protein samples immobilized on a nitrocellulose or polyvinylidene fluoride (PVDF) membrane. The WesternSure Pre-stained Chemiluminescent Protein Ladder is visible on the membrane and reacts with chemiluminescent substrate to emit light. This formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture. The WesternSure Pen can be used to annotate any visible protein ladder (such as the Chameleon Vue Ladder) prior to chemiluminescent Western blot detection. Two versions of the MW marker are offered. The protein markers can then be visualized using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel). The ladder’s recombinant proteins bind to the antibodies used in the western blot through the IgG binding site. The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.Ĭompare and view all other protein standards and ladders › Thermo Scientific SuperSignal Molecular Weight Protein Ladder (20 to 150K) is a mix of eight unstained recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems.
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